Reverse Transcriptase RNAscribe RT


Quality level: 100


  • enough for 25 reactions
  • enough for 50 reactions
  • enough for 500 reactions


dNTP included: no
hot start: no


  • 200 reaction pack (03531287001)
  • 25 reaction pack (03531317001)
  • 50 reaction pack (03531295001)


RT-PCR: adequate
RT-qPCR: adequate

Detection method: probe-based

General description

In retroviruses such as human immunodeficiency virus type 1 (HIV-1), reverse transcriptase (RT) is the main enzyme. HIV-1 RT is made up of two subunits of 66 kDa and 51 kDa (p66 and p5l). A human endogenous retrovirus of the HERV-K family encodes the enzyme reverse transcriptase (RT). A recombinant reverse transcriptase for robust transcription of RNA fragments up to 14 kb was used in two-step RT-PCR in thermal cyclers and real-time PCR instruments.


Transcriber Reverse transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (eg, LightCycler® instruments) for the following applications:

  • Synthesis of first-strand cDNA for use in subsequent amplification reactions.
  • RT-PCR of GC-rich RNA templates
  • Cy3, Cy5, DIG, biotin, and aminoallyl labelling during cDNA synthesis
  • Recovery and cloning of the 5 ′ and 3 ′ ends of mRNA by RACE
  • Generation of cDNA libraries with large inserts.
  • Dideoxy DNA sequencing
  • RNA sequencing
  • Marking the 3 ‘end of DNA fragments
  • Generation of single-stranded probes for genomic fingerprints
  • In reverse transcription of human papillomavirus E6 RNA, cortical and striatal tissues, muscle biopsies from
  • Becker muscular dystrophy specimens, and human brain microvascular endothelial cell and oocyte miRNA stem-loop (HCMC)

Biochemical / Physiological Actions

The reverse transcriptase of human immunodeficiency virus type 1 (HIV-1 RT) is essential for the catalytic conversion of single-stranded viral RNA into linear double-stranded DNA that is integrated into host cell chromosomes.

Features and Benefits

  • Achieve high sensitivity in two-step RT-PCR.

Reverse Transcriptase Transcriptor is used in conventional thermal cyclers and real-time PCR instruments (eg LightCycler® instruments).

  • Get more complete transcripts, up to 14 kb.

Large insert cDNA libraries can be generated.

  • Transcribe difficult templates in reverse.

The enzyme works well at elevated temperatures, thereby overcoming the secondary structure of RNA (eg, GC-rich RNA templates) and providing optimal reaction conditions.

  • Efficiently label the cDNA.

Nucleotides labelled with Cy3, Cy5, DIG, biotin, or aminoallyl are incorporated during cDNA synthesis.


  • Transcriber Reverse Transcriptase, in storage buffer
  • Transcriptional Buffer RT, 5x concentrated


Each lot of Transcriptor Reverse Transcriptase is routinely tested in RT-PCR:

  • with a conventional thermal cycler to detect a 10 kb fragment of the human dystrophin gene, from total RNA of human skeletal muscle
  • with the LightCycler® instrument to detect 5×102 to 5×106 copies of human PBGD RNA transcribed in vitro. Results are defined at fixed crossover points and fixed fluorescence intensity.

Preparation note

Reverse transcriptase transcriber is recommended for RT-PCR of:

  • long targets, because it can transcribe RNA templates up to 14 kb
  • rare objectives, because it has a high sensitivity
  • GC-rich lenses, because can operate at high temperatures (up to + 65oC) to eliminate the problems associated with a large secondary structure.

Labelling for many applications

Transcriber reverse transcriptase is also recommended for preparing labelled cDNA, as it accepts a wide variety of modified nucleotides (including Cy3, Cy5, DIG, biotin, or aminoallyl-labelled dNTPs).

Reaction requirements

Transcriptionist accepts ssRNA and ssDNA templates and requires a primer for transcription.

Other notes

For life science research only. It should not be used in diagnostic procedures.

Legal information

LightCycler is a registered trademark of Roche

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