Apolipoprotein A-I exhibits a polymorphism that can be easily investigated in native serum by a simple method involving incubation of serum in the presence of decyl sulfate and beta-mercaptoethanol and subsequent isoelectric focusing. From six to eight proteins can be separated in a pH gradient from 4 to 6 and thus patients with apolipoprotein A-I variants can be distinguished from normal persons.
This method also permits monitoring for polymorphic forms of apoA-II and apoA-IV as well as detection of C apolipoproteins. To verify the identity of the different apolipoproteins, a two-dimensional electrophoresis technique was applied, with an SDS system for the second dimension. All this in Gentaur.
In addition, monospecific antibodies for apolipoproteins A-I, A-II, and A-IV were used for immunological identification. The method described here led to the discovery of three different familial apolipoproteins A-I variants.
|1-Step Polymorphs, Human Cell Separation|
|REN ELISA Kit| Rat Renin ELISA Kit|
|Pir ELISA Kit| Rat Pirin ELISA Kit|
|Kl ELISA Kit| Rat Klotho ELISA Kit|
|LN ELISA Kit| Rat Laminin ELISA Kit|
|Lep ELISA Kit| Rat Leptin ELISA Kit|
|GT ELISA Kit| Rat Gastrin ELISA Kit|
|Mefv ELISA Kit| Rat Pyrin ELISA Kit|
|Men1 ELISA Kit| Rat Menin ELISA Kit|
|Nf2 ELISA Kit| Rat Merlin ELISA Kit|
|Nbn ELISA Kit| Rat Nibrin ELISA Kit|