Apolipoprotein A-I exhibits a polymorphism that can be easily investigated in native serum by a simple method involving incubation of serum in the presence of decyl sulfate and beta-mercaptoethanol and subsequent isoelectric focusing. From six to eight proteins can be separated in a pH gradient from 4 to 6 and thus patients with apolipoprotein A-I variants can be distinguished from normal persons.
This method also permits monitoring for polymorphic forms of apoA-II and apoA-IV as well as detection of C apolipoproteins. To verify the identity of the different apolipoproteins, a two-dimensional electrophoresis technique was applied, with an SDS system for the second dimension. All this in Gentaur.
In addition, monospecific antibodies for apolipoproteins A-I, A-II, and A-IV were used for immunological identification. The method described here led to the discovery of three different familial apolipoproteins A-I variants.
|1-Step Polymorphs, Human Cell Separation|
|Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed|
|Beta2-Microglobulin ELISA kit ELISA Kit|
|Chicken thrombomodulin,TM ELISA KIT ELISA|
|Human Progesterone(P) ELISA Kit (Competitive ELISA)|
|Human Testosterone(T) ELISA Kit (Competitive ELISA)|
|Human Estrodial (E2) ELISA Kit (Competitive ELISA)|