1-Step Polymorphs, Human Cell Separation

Apolipoprotein A-I exhibits a polymorphism that can be easily investigated in native serum by a simple method involving incubation of serum in the presence of decyl sulfate and beta-mercaptoethanol and subsequent isoelectric focusing. From six to eight proteins can be separated in a pH gradient from 4 to 6 and thus patients with apolipoprotein A-I variants can be distinguished from normal persons.

This method also permits monitoring for polymorphic forms of apoA-II and apoA-IV as well as detection of C apolipoproteins. To verify the identity of the different apolipoproteins, a two-dimensional electrophoresis technique was applied, with an SDS system for the second dimension. All this in Gentaur.

In addition, monospecific antibodies for apolipoproteins A-I, A-II, and A-IV were used for immunological identification. The method described here led to the discovery of three different familial apolipoproteins A-I variants.

1-Step Polymorphs, Human Cell Separation
Rat Cholesterol ELISA ELISA
Goat Cholesterol ELISA ELISA
Mouse Cholesterol ELISA ELISA
Human Cholesterol ELISA ELISA
Sheep Cholesterol ELISA ELISA
Monkey Cholesterol ELISA ELISA
Canine Cholesterol ELISA ELISA
Rabbit Cholesterol ELISA ELISA
Bovine Cholesterol ELISA ELISA
Porcine Cholesterol ELISA ELISA

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